猪圆环病毒2型感染仔猪肺泡巨噬细胞Toll样受体mRNA转录的变化

doi:10.11843/j.issn.0366-6964.2014.05.018

Abstract

Abstract:

Six conventional piglets free of porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) were used in this experiment as detected by ELISA and fluorescence quantitative PCR.The alveolar macrophages (AMs) were isolated aseptically and divided into two groups，control group and PCV2 infection group (PCV2 group) and cultured in vitro.The AMs were collected at 0，6，12，24 and 48 hours post infection (hpi).The amount of PCV2 infected cells was observed by indirect immunofluorescence assay (IFA) and the mRNA transcription of TLR2，TLR3，TLR4，TLR7，TLR8 and TLR9 were detected by real-time PCR.The results show that the mRNA levels of TLR2 were significantly higher at 6，12 and 24 h (hpi) (P<0.01 or P<0.05)，then the levels were recovered at 48 hpi.The expression of TLR3 were significantly elevated at 6 and 12 hpi (P<0.05)，and the transcription were then restored at 24 and 48 hpi; PCV2 stimulation up-regulated the transcriptions of TLR4 in AMs at 12 hpi (P<0.05) and down-regulated the transcriptions at 24 and 48 hpi (P<0.05); PCV2-infection increased TLR7，TLR8 and TLR9 transcriptions in AMs at 6 and 12 hpi (P<0.01 or P<0.05) and their of transcriptions were restored at 24 and 48 hpi.Thus，PCV2 can significantly affect TLR2，TLR3，TLR4，TLR7，TLR8 and TLR9 transcriptions in piglets AMs in vitro.

CLC Number:

S858.285.3

ZHANG Ya-qun， HAN Jun-yuan， GUO Hua， CHEN Meng-meng， DUAN Dian-ning， ZHANG Shu-xia. Changes of Toll-like Receptor mRNA Transcription in Alveolar Macrophages of Piglets Infected with Porcine Circovirus Type 2[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 802-808.

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Changes of Toll-like Receptor mRNA Transcription in Alveolar Macrophages of Piglets Infected with Porcine Circovirus Type 2

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